FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004723288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26598992
Sequences flagged as poor quality0
Sequence length8-384
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG4952511.8619164214944688No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC4548581.710057283373746No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC4116811.547731583211875No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG2996661.1266066022351524No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG2613810.9826725764645516No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA1633310.6140495850369067No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1568560.5897065573011188No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA1458030.5481523510364603No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG1391700.5232153158285096No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA1247190.46888618937138665No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC1032520.3881801235174626No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG957510.35997980675357927No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT784750.295029977075823No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT681020.2560322586660427No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC621540.23367050901778533No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA613840.23077566247623219No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA587870.22101213459517563No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC545660.20514311219011608No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC544320.20463933370106654No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGTTC528890.19883836199507107No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC476250.17904813836554406No Hit
TGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTC467040.17558560113856947No Hit
TATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC460690.17319829262702888No Hit
GATCATTTCTATAGTGTGTTGCTAGAGAAGTTTCTCTGAACGTGTAGAGC451290.1696643241217562No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG419870.1578518464158341No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC402040.1511485848787052No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC398310.1497462760994853No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA381050.14325730839725054No Hit
AAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAATAGCCACTGCACTCC374640.1408474426399316No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG370140.1391556492065564No Hit
TGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA369350.13885864547047497No Hit
GTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAG362020.13610290194455488No Hit
GTGGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGT340980.12819282775828497No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT339940.12780183549812715No Hit
GAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG339750.1277304042198291No Hit
TAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCC338450.1272416638946318No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACCA335050.12596341996719274No Hit
TCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGC308460.11596680054642673No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC296930.11163204981602311No Hit
GTAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCA296710.11154933991483586No Hit
TCTGCCCCATGATGTACAGTCCCTTTCCACAACGTTGAAGATGAAGCTGG288000.10827477973601406No Hit
TTAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCA287270.10800033324571097No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT276910.10410544880798492No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGC270550.10171438075548125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)