FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004723289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22208161
Sequences flagged as poor quality0
Sequence length8-379
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTT1321270.5949479562940849No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG854470.38475495562194456No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT667650.300632726861085No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC629140.28329225459055346No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC610400.27485391518910546No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC542400.2442345406267543No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA521560.23485060289323373No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGTGTCTGGAACTCTGAGGT513610.23127083777895885No Hit
TTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCG491520.22132404389539503No Hit
TTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAG481530.21682569754424963No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC464670.20923389379246662No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC462060.20805865015117642No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA452140.2035918237444334No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCG441470.1987872836476645No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGC441440.19877377510006344No Hit
TACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT424060.1909478231898625No Hit
GGCAGATGATGTTTGTTTTCACGATGGTCTTCAGATGCCCACGTGGGCAC413130.18602620901388456No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG407340.18341905932688438No Hit
TGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAAT405870.18275714049443356No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG400230.18021753354543854No Hit
CGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTC396490.17853346794450925No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAAC386240.17391804751415482No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC383770.1728058437616694No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT383730.17278783236486803No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT378330.17035629379668132No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA377250.169869986083044No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG368530.16594350158034246No Hit
TTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGG367930.1656733306283217No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC358350.1613596010943905No Hit
GATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATG344100.15494304098389777No Hit
AGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGG343540.1546908814286784No Hit
TACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCG327800.14760339678733417No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC327220.14734223153371412No Hit
GGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT325090.14638312465404046No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC296420.13347345599664914No Hit
TGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGA293240.1320415499509392No Hit
AAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGAT291350.13119051145207386No Hit
TCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCT274680.12368426183509748No Hit
GCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGG273210.12302234300264664No Hit
GTGCTGTGATGATGCCTTAATATTGTGGTTTCGACTCACTGAGAGTAAAA269690.12143734008412492No Hit
AGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGT269440.12132476885411629No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC262600.11824482000107979No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG260500.11729922166900718No Hit
CGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAA256190.11535849366365815No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG251480.1132376516902953No Hit
CATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGT250200.11266128699265103No Hit
TGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTC249510.11235059039782717No Hit
GACCAATGATGAGACAGTGTTTATGAACAAAAGATCATGATTAATCCAGTTCTGCACAAAACACTGAGGT243280.10954531534601175No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA242460.10917608171158343No Hit
GTCTAATGATGAATTCATAGGGCAGATTCTGAGGTGAAAATTTAATTCAT239890.10801884946709454No Hit
GTCTAATGATGAATTTCATAGGGCAGATTCTGAGGTGAAAATTTAATTCA234040.10538468268489229No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT231750.1043535302180131No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCC230610.10384020540917369No Hit
CGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGC227140.1022777167366537No Hit
TGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGG224540.10110697594456379No Hit
GAGCGTGATGATTGGGTGTTCATACGCTTGTGTGAGATGTGCCACCCTTG224300.1009989075637555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)