FastQCFastQC Report
Wed 22 Mar 2023
EGAF00004723290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56519575
Sequences flagged as poor quality0
Sequence length8-384
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG17312623.063119282124821No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC16138482.8553788665254474No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGT14669712.5955096088390617No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC11077851.9600023531670223No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA10725471.8976558121677312No Hit
TGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA10510651.8596477415125645No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGC9209871.629500929545206No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG6390031.1305870576698427No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT6198201.0966466042959453No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAA6188661.094958693514592No Hit
GAGCATGGTAATGGATTTATGGTGGGTCCTTCTCTGTGGGCCTCTCATAG4312300.762974597738925No Hit
AGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCG4041690.7150956106800166No Hit
TCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAAA3572460.6320748165569186No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT3109840.5502235287508797No Hit
AGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTG3076610.5443441497923507No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC3064210.5421502196362942No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG2553820.45184699283389873No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT2481070.4389753461521959No Hit
ACACCCAACAGGAATCTGGGGTCATTGTGATGAGGGCGTCAAACTTGTGG2426680.42935213154026725No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGGAGTTCTGGGCTGTAG2322330.41088950155764614No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAG2287860.4047907295835116No Hit
GAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTG2265790.40088588776543355No Hit
GACCTCCTGGGATCGCATCTGGAGAGTGCCTAGTATTCTGCCAGCTTCGG2241200.39653518272209226No Hit
GACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGA2104070.37227279221402493No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG2019080.35723552415247284No Hit
AGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCG1826410.32314644970348766No Hit
AAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGATGTGCTG1757790.31100552330763276No Hit
TGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCT1752560.31008018018536054No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA1649890.2919147923529149No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTGCTGGGCTGT1570760.2779143332199508No Hit
CCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTCTG1451290.25677652388575106No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1444070.25549909035940205No Hit
AGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGC1414560.25027789044769No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG1391730.24623858194262782No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGACCTGG1367270.241910877779955No Hit
GAGAGTGATGAGTTGCACACTGGTGGAGCCATGGTATCAGGTGATACAGG1348900.238660676411668No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG1342340.23750001658717357No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA1325620.23454174947352313No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATTGTTCACAGTCAGTT1265540.22391180400772653No Hit
ACAATATATTAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGC1265490.2239029575151618No Hit
GGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAG1253250.2217373361353124No Hit
GACCTCCTGGGATCGCATCTGGAGACTGCCTAGTATTCTGCCAGCTTCGG1241530.21966371827813638No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG1205730.21332962960177956No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC1188670.21031120633868886No Hit
AGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGGAGTTCTGGGCTGTAGTG1138100.2013638637587066No Hit
CGGATGACTTGTGGCTGGGGGTGAAAGGCCAATCAAACCGGGAGATAGCT1097670.1942105898708545No Hit
TGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACGCATCGA1087890.19248021592519052No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT1073800.18998727432044563No Hit
ATAGGGCGGAGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTG1038440.18373103477865854No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGTTC1023460.1810806256062612No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCC1002050.17729255749003775No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGGAGTCCAGGAGTTCTGGGCTGT992800.17565595636556006No Hit
AGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGGAGTTCTGGGCTGTAGTG991930.1755020273949335No Hit
GAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTCTGGCGTTGCTTGGC959370.16974119143677213No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGG958060.16950941333157582No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC935830.16557626273729056No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC909440.1609070839616186No Hit
AGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAAT891740.15777542559369917No Hit
GGATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCAT882310.15610697709598842No Hit
GTGGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTCTGT860950.1523277554723297No Hit
GATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCACG860340.15221982826303984No Hit
GGAGCGGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT844320.14938541204529582No Hit
CGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGA833340.14744272227807798No Hit
GAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTG796210.14087331689949897No Hit
GATGTGCTGACCCCTGCGATTTCCCCAAATGTGGGAAACTCGACTGCATA788630.13953218862668376No Hit
GAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATC779310.13788320241261545No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG779160.13785666293492121No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT775370.13718609879851362No Hit
TCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGC764350.135236331837244No Hit
GGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGT760800.13460823086514714No Hit
TAAATGGATTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACT751300.13292739727784578No Hit
AGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAATAGC734840.130015131925532No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC728140.12882970192185628No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC724670.12821575533786303No Hit
AGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTCTGGCGTTGCTTGG721610.1276743499929007No Hit
GTAGGGTGATGAAAAAGAATCCTTAGGCGTGGTTGTGGCCGTCTTGGTCA707110.1251088671491249No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG679130.12015836990989404No Hit
GAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTGG671360.1187836249653328No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC669190.1183996871880229No Hit
GGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGT665930.11782289587280159No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA645860.1142719137573133No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGG625570.1106820070745401No Hit
GTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGA622040.11005744469946917No Hit
TGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATAT613910.10861900500844177No Hit
TTAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCA610940.10809352335009595No Hit
AAAATCAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACA602870.10666569945014626No Hit
TGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG590560.10448769298070625No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGG589150.10423822189038046No Hit
GGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGT588670.10415329556175891No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG585880.10365966127664618No Hit
GGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAA580670.10273785675139985No Hit
CGGGAGGCTGAGGCTGGAGGAGTCGCTTGGAGTCCAGGAGTTCTGGGCTG579770.10257861988523444No Hit
AGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTCTGGCGTTGCTTG575000.10173466449455787No Hit
AGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTG571680.10114725738825883No Hit
ATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAG569530.10076685820797485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)