FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004723291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70944390
Sequences flagged as poor quality0
Sequence length8-384
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTT16886682.3802699551014532No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC10129611.4278239618382793No Hit
GGAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTT7752521.09276011817143No Hit
TACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCG7315381.0311428430070368No Hit
TTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCG7151561.0080515175336626No Hit
AGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGG6890740.9712875112464847No Hit
TTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGG5573020.7855476662777704No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT4027250.5676629258493872No Hit
TCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCT3799400.5355462214841794No Hit
GAACCTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGGGTCGGGGTTT3776610.5323338462702971No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCT3616350.5097443222783365No Hit
CGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTC3568440.5029911456000961No Hit
TACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTA3373210.475472408741551No Hit
TTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAG3368060.47474648805916864No Hit
TAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAA3356610.4731325479012506No Hit
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG3186480.44915179339761746No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA3141110.4427566436190374No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCG2825640.39828942077026813No Hit
GATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATG2548240.3591883727522359No Hit
TGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAAT2529000.3564763894650444No Hit
GGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTC2490620.351066518437892No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2431840.3427811557756716No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA2242540.3160982848679085No Hit
TGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGG2013290.28378424284147064No Hit
GGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT1937630.27311955180670383No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1883850.2655389665060197No Hit
AGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGT1846350.2602531362944977No Hit
TAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCC1804020.2542864911517317No Hit
AAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGAT1743410.24574317997518902No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC1640010.231168384138619No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG1632830.2301563238474529No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC1584800.2233862325125355No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCC1583290.22317338974935158No Hit
TAAATGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCAC1546760.2180242863459676No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGAATAGGAGCTTGCTCCGTCCAC1539910.21705874136066292No Hit
TACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGT1532950.21607769127340443No Hit
ATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTG1528560.2154588967499756No Hit
TGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGA1515270.21358559852301218No Hit
GCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGG1501870.21169679519409496No Hit
CATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGT1431100.20172137641891064No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC1414790.19942239266557932No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC1409480.1986739191076278No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAAC1394330.19653844370217294No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA1383020.19494423731037788No Hit
TGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTC1339240.18877320673276632No Hit
AAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACC1310900.18477852864757877No Hit
GGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC1302650.1836156460010439No Hit
TGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGG1282720.18080640343796037No Hit
CGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAA1281120.18058087468226874No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT1270770.17912198554388867No Hit
CGACCTGCTTCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGC1270560.17909238489470414No Hit
GGCTGGTCCGAGTGCAGTGGTGTTTACAACTAATTGATCACAACCAGTTA1264930.1782988055856143No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG1261860.17786607228563103No Hit
TGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATC1235120.17409692295613508No Hit
ACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAG1201270.16932558021853455No Hit
TGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCG1177230.16593701066426816No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1149530.16203254408135725No Hit
AATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCC1144160.1612756131950673No Hit
CTACCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAAT1130300.15932197034888876No Hit
CGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCAT1127250.15889205615835164No Hit
TGGATTTTTGGAAATAGGAGATGGAATAGGAGCTTGCTCCGTCCACTCCA1124210.15846355152253758No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA1102800.15544569486043927No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1018560.14357160587327625No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAG1015060.14307826172020086No Hit
TAGATTGAGGCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGT1013790.14289924827037065No Hit
AGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGT987980.13926118753012043No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA967310.1363476379175295No Hit
AGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA960750.1354229700191939No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAAT944720.13316345379810862No Hit
GTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATA928640.130896889803408No Hit
CGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGT928240.1308405076144851No Hit
TGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGA926710.13062484574185498No Hit
CAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTG889350.125358749296456No Hit
GAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTG864520.1218588249190669No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC859340.12112867557251533No Hit
GAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTT830160.11701559489058966No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTATTG830050.11700008978863587No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA829600.11693665982609759No Hit
ACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCC813670.11469123915224305No Hit
TCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTA807830.1138680591939687No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCA798280.1125219344334344No Hit
AGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGG794130.11193696922335931No Hit
AGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTGCCCTCGGCCGATC793690.11187494881554412No Hit
CGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGG790990.11149436904031454No Hit
GACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC783740.11047244186608696No Hit
TGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCT760470.10719240802549715No Hit
TCCCTACCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGG741390.10450297761387475No Hit
TGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTC736220.10377423782204624No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATGGCTTTCTCACCA727290.10251550545434247No Hit
AGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA725990.10233226334034305No Hit
ACAGGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTT719640.10143719609119199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)