FastQCFastQC Report
Sun 12 May 2024
EGAF00004723296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40060983
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT7573831.8905751763505154No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT2617020.6532590575723017No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT2337590.5835078984457271No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1430420.3570606342834873No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1423980.3554530851127642No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA1377350.34381333079120896No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1304130.32553619565451003No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG912580.2277977053134218No Hit
AGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTG778160.19424386066612492No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC678960.1694816125705153No Hit
CTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCACCTCAGCCATTGA586060.14629196692452603No Hit
TTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTT568190.14183126759520603No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG565380.14112983697878806No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC512640.12796490790053755TruSeq Adapter, Index 9 (100% over 50bp)
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC491550.12270043398585602No Hit
TGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTG449860.11229379968035233No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC424960.10607827571280516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)