FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13258866
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT564050.4254134554191889No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA530170.3998607422384388No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG312020.23532932605246937No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG284900.2148750881108535No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA230440.17380068551865596No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC210630.15885973958858923No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT205680.15512638863685627No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT169610.12792195048958183No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA166360.12547076047076725No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC162520.12257458518699865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG42800.036.3798831
TGGAGTC209500.029.8863432
GGAGTCT209600.029.7823093
GAGTCTT232600.026.7157214
TGCGCGG23550.025.9212447
AGTCTTG251150.025.4355075
GTGCGCG24800.024.6147276
CGCGGAC24200.024.0613239
GTCTTGG266700.023.5578946
CTCGCTA25550.023.198331
TCGCTAT28250.021.4807762
TATACCC279000.020.64284194
TCTTGGA319200.020.0804357
CTTGGAA312250.019.7906518
CTGGAGT491750.019.4860481
TTGGAAG320300.019.3521929
GCTATGT87400.018.2997932
GGAGTGC308500.018.0233755
CGCACTA103850.017.37486378-79
GAGTGCA323250.017.1414226
Produced by FastQC (version 0.11.5)