FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10411685
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT194950.187241546397149No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA142880.1372304290804034No Hit
GTTTTCTCATGCAACACACATAACTGGTTCAGGACCACGGATAGATAGTC121520.11671501779010793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG15550.032.7484631
TCGCGCG3900.025.3118069
GACCGCG5750.019.6893545
TATACCC104550.019.05504694
TGGAGTC111850.019.054242
GGAGTCT111950.019.0371273
GAGTCTT115750.018.4614264
ACCGCGT5900.018.3250126
AGTCTTG121500.018.2865875
ACCGAAG48800.018.00486894
CTGGAGT204850.015.9282351
CGCGGAC4051.0453732E-815.0888729
CTCGCGC6550.015.0711528
ATGCAAC86500.014.6729059
TGCGCGG4251.9732397E-814.3788087
TCTTGGA158700.014.0400897
CGCACTA28500.014.01574878-79
CTCGCTA10500.013.9209841
CCGCGTT8150.013.842747
TCGCTAT11250.013.8309052
Produced by FastQC (version 0.11.5)