FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20073892
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG572910.28540055909436995No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT492340.24526384818649016No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG300070.14948272113848177No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC278590.13878225508038003No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA253850.12645778905256638No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA211700.10546036613129134No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG205820.10253118827181096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC54450.048.0675961
GCGATCT74050.035.2811432
GGGCGAT30850.029.7012351
CGATCTG89600.029.5251583
CGCGTGC42400.028.4814661
GGCGCGT19400.021.7989651
GCGTGCC59200.020.7956492
TCACCCC332450.017.7099193
GGCTGCG161450.017.1952369
GTCACCC335200.017.186182
CGCGAGT8450.016.6824421
CCCCATT353050.016.4357326
CCCATTG347600.016.4230887
TGTCACC360850.016.2511061
ACCCCAT358600.016.1551935
CACCCCA384350.015.5618384
ACTCCGC25250.015.2584995
ACGCACG29250.014.9344794
CGTAGGG81750.014.8015780-81
CCATTTG328000.014.7644694
Produced by FastQC (version 0.11.5)