FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004723312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61799043
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT10804971.7484040974550368No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT5355470.8665943257406106No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT4383180.709263410438249No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG3571270.5778843533224293No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG3071210.49696724268044085No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2781280.45005227669949516No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA2191760.3546592137357208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2180610.35285497867661153TruSeq Adapter, Index 9 (100% over 50bp)
AGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTG1868540.3023574329460086No Hit
TTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTT1481720.23976423065321578No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG1379720.2232591206954451No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC1255170.20310508691857898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1086600.1758279654913103No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG1082690.17519526960959572No Hit
TGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTG1026850.1661595309817338No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC985230.15942479885974933No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC919030.1487126588675491No Hit
TGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGA867340.14034845167424356No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC816230.13207809706697238No Hit
CTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGA726410.1175438914159237No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA698670.11305514876662409No Hit
GCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGG696150.11264737546178506No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC671520.10866187685139397No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG621280.10053230112317434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)