FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13443798
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG223190.16601707344903574No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT157000.11678247471436272No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT156190.11617996640532684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC57400.043.2281381
GGCGCGT21900.041.6295851
GGCGATC18950.037.1985971
GCGTGCC72650.034.6067662
GCGCGTG30200.030.188232
GCGATCT23550.029.9325182
CGTGCCT99850.024.9908643
CGATCTG34350.020.7946513
GCTGCGA66100.020.0485274
GGCTGCG68550.019.5376433
CTGCGAC78050.017.1595865
GCGACAT78450.017.1317397
CCTGTAG151550.016.7752747
GCCTGTA159600.016.2235346
CGTAGGG31850.016.17495580-81
TGCGACA83100.015.9468476
TGTAGTC157650.015.8828169
TGGCGCG15450.015.2084312
CGACATC87950.015.1704828
TCACCCC170100.014.86321453
Produced by FastQC (version 0.11.5)