FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579990
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT112650.10647458078882872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC37200.043.4576841
CGCGGGT7750.032.138471
GGCGCGT13200.031.685981
GCGTGCC51400.031.4518642
CGTGCCT76850.021.392513
GCGCGTG20300.020.6036912
GGCGATC11050.019.5635031
GCGGGTC14200.017.5403632
GCGATCT14200.015.88561252
CCTGTAG111550.014.9905647
CGGGTCT17050.014.6012173
TCGTCGG11450.014.57661514-15
GCCTGTA118950.014.4133846
CGCTAAT6003.6379788E-1214.0984811
TGTAGTC119650.013.9827469
CGCGAGT3559.400255E-613.238011
TGGCGCG10800.013.054152
TTCGATC9150.012.326542
CGCGGTG9500.011.8724051
ACTCCGC11100.011.8487535
Produced by FastQC (version 0.11.5)