FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19250165
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG270460.140497497034441No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC254560.13223782757186756No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT214700.11153151154808284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT36850.040.6580161
CGCGTGC90100.039.5648881
GCGTGCC112700.031.7976282
GGCGATC23450.031.0442961
GCGCGTG51000.029.8379482
GCGATCT30950.022.4592462
CGTGCCT160650.022.3370553
ACTCCGC23150.020.7800335
CGATCTG43100.016.0196633
TGGCGCG27850.015.1785613
GCCTGTA249900.014.6451076
TCCGCTG44400.014.5523647
TCACCCC214200.014.4942353
CCTGTAG251550.014.4927617
CGCGGGT8450.014.451391
CCCATTG194050.014.4124227
ACCCCAT207100.014.16475
CGTAGGG51600.013.88517580-81
CAGCGCA49650.013.773449
CCCCATT203900.013.7624846
Produced by FastQC (version 0.11.5)