FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16432526
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG649630.39533179500218046No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT410290.24968163750357067No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT385020.2343036000673299No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC334370.20348058478653863No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA311550.18959349280810509No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG301010.18317938459370156No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA299820.18245521108562374No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA268730.1635354175006326No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG235920.1435689193493441No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG227420.13839625143450252No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC226080.1375807955514569No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG224790.13679576712673389No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC187120.11387172002648893No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC183830.11186959326886202No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT166120.10109218753099798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC42000.052.1651421
GGCGCGT59150.042.206911
CGCGTGC142550.040.6008031
GCGATCT55100.039.9335632
GCGTGCC176000.032.99122
GCGCGTG82800.030.2082162
CGATCTG74800.029.7934323
CGTGCCT219650.026.499233
GCTGCGA148500.020.4843244
GGGCGAT39050.020.3474251
ACTCCGC24800.019.9059435
GGCTGCG154300.019.8666883
CCTGTAG301150.019.4211967
GCCTGTA310400.019.2059636
ACGCCGC14000.019.1421452
GTGCCTG310950.018.8698234
TCACCCC339200.018.6427613
CACGCCG15000.018.1794411
TGCGACA167750.018.1054156
GTCACCC347500.017.7104112
Produced by FastQC (version 0.11.5)