FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19347539
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG795440.4111323926004233No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT448150.23163152688308314No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT443950.22946070815518194No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA388390.20074387755465956No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT320460.16563346893886607No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA302150.15616973300842033No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG298460.1542625136974785No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA284940.14727454484004399No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG270310.1397128596045213No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC263300.1360896597753337No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG248850.1286210096281496No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG212030.1095901654468819No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC203700.10528470830321107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC49900.052.1784061
CGCGTGC157000.047.0880471
GCGTGCC182250.040.71862
GGCGCGT56200.038.6355631
GCGATCT67150.038.6341442
CGTGCCT239850.030.9769363
CGATCTG92050.028.2145463
GCGCGTG80050.027.4765852
CGACCTA14750.022.2883424
GCCTGTA347750.021.8649836
GCTGCGA181350.021.7019214
CCTGTAG347650.021.5605647
GGCTGCG187500.021.1403853
GTGCCTG361950.020.7348024
CGCGAGT9600.019.58261
TGTAGTC383650.019.5147139
TGCGACA203850.019.2603876
TGCCTGT399650.018.9668585
CTGCGAC208050.018.9393885
GTCACCC422400.018.5810722
Produced by FastQC (version 0.11.5)