FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13821724
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG375320.2715435498494978No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT313160.22657086771519963No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC265350.19198039260514824No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT249370.1804188826227466No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA214310.15505301654120718No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG185050.13388344319420645No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG178580.12920240629895374No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT170700.12350123616995969No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA162740.11774218614117893No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA149870.10843075726298687No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG145620.10535588758681623No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC143860.10408252979150791No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC143500.10382207024246758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC26100.045.748721
GGCGCGT46050.042.7730371
CGCGTGC115300.042.4838751
GCGTGCC138700.035.5198022
GCGATCT37100.031.6775652
GCGCGTG63650.031.388912
CGTGCCT186050.026.5808333
CGATCTG44800.026.3377253
CGACCCG7350.021.1061573
ACTCCGC16000.020.8602435
GGGCGAT22800.020.6182041
CCTGTAG262650.018.8643177
GCCTGTA276950.018.3994886
GTGCCTG275750.018.0023574
TCACCCC216650.017.4670583
GCTGCGA85700.017.333544
TGTAGTC294650.016.848999
CGTAGGG64900.016.82842680-81
CGCGGTG14250.016.8244551
TGGCGCG32800.016.7685153
Produced by FastQC (version 0.11.5)