FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18943980
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1487880.785410457570162No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA811980.4286216518387372No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT758190.4002274073346784No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT549830.290239960135093No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG493460.26048380540942295No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA469880.24803657943051038No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG459170.24238306839428672No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA412070.21752028876719673No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG289660.15290345534570876No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC288440.15225945128742746No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC274980.14515429175917627No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC261940.13827083854607108No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC203140.10723195442562757No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC201150.10618148878957855No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC195560.10323068330942073No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT195490.10319373225689639No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG192990.10187405180959862No Hit
CGAAGACCGGTCCTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCG192560.1016470667726634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG69900.042.0490261
TGGAGTC398900.039.294042
ACCGCGT12600.036.8866926
GGAGTCT437750.035.8817333
GACCGCG13900.033.8340845
CCGCGTT13700.033.582317
GAGTCTT479250.032.6964454
AGTCTTG507200.031.3498275
GTCTTGG526850.030.0383996
CTCGCTA36500.028.4742621
TCTTGGA585800.027.0236037
CGCGTTC18400.025.5145788
TCGCTAT40750.025.5046922
CTGGAGT830550.025.321481
TGCGCGG45050.025.1146587
CTTGGAA622300.025.114258
CGCGGAC45250.025.0039849
TTGGAAG652150.023.9649819
AGACCGC20350.023.5718384
GTGCGCG48950.023.2096026
Produced by FastQC (version 0.11.5)