FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19750190
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT226310.11458623942351948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC98700.036.681521
GGCGCGT46350.032.0561261
GCGTGCC119750.030.2726442
GCGCGTG61100.024.3943672
CGTGCCT163250.022.4651663
CGCGGGT14700.018.8715841
GGTCGAC12200.018.1138465
TGGCGCG38200.015.8779543
CGACCCG14100.014.9944128
CCTGTAG258350.014.8940797
GGCGATC18300.014.1314041
TGTAGTC275100.013.8676069
GCCTGTA303800.013.6661646
GTCGACC17700.013.2822136
CGTGCGG17800.013.19732958
GCGACAT33750.013.0855617
CGACCAT70750.012.6934791
GTGCCTG301150.012.4747584
ATCCCCG78250.012.25799756
TGCGACA36300.012.1757296
Produced by FastQC (version 0.11.5)