FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15073478
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1298010.861121766323605No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1132460.7512930990445602No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT834990.5539464747286592No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA552750.36670368975229206No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG520930.3455937640934627No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG514780.34151375017762986No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT483800.32096109471218254No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA457610.30358620618280663No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC436960.28988664726216473No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG364160.24158989716905416No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC322700.2140846326242689No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC257140.1705910208645941No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC229300.1521214944553606No Hit
AGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTG209460.1389593032211942No Hit
CGAAGACCGGTCCTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCG200060.13272318439049038No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC194270.12888200055753557No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC185600.1231301760615566No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC181410.12035045926361519No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA174470.11574634599924451No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC164690.10925812874772498No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC163070.1081833933747739No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG156760.10399723275543972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG68100.053.3981821
ACCGCGT10800.049.1477626
GACCGCG10950.048.0453645
CCGCGTT10850.047.189557
TGGAGTC313050.040.8664172
GGAGTCT348400.036.69943
AGACCGC15400.034.162134
GAGTCTT387550.032.9313474
CGCGTTC16300.031.9878048
TGCGCGG28000.031.539077
AGTCTTG418250.031.0307675
CTCGCTA28700.030.2846431
GCTATGT121500.030.2386972
CGCGGAC29100.030.0241329
GTGCGCG29650.029.942376
TCGCGCG31000.029.5476829
GTCTTGG442050.029.0201326
TCGCTAT31500.027.4435252
CCTCGCG33550.027.44187
TTATGCG17700.026.8038488
Produced by FastQC (version 0.11.5)