FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37412438
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3136260.8382934039209099No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2011180.5375698851809657No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA699810.18705276571390508No Hit
CCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGC632520.16906676865057552No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT605500.16184457158338625No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA533460.1425889432813761No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA468550.12523909829132227No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA468250.12515891105519505No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA465120.12432229089160134No Hit
GCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGA459320.1227720043264756No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC447920.11972488935364223No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGG425530.11374024863068266No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT421820.11274859981057637No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG407620.10895307063388919No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT406240.1085842093477041No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG403460.1078411409595921No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA395740.1057776560832523No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT393940.10529653266648914No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC385000.10290695302989877No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG383510.1025086897571337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG56450.048.440422
TCGGAAG188150.047.75525394
CGTACGG59450.046.4107253
ACCGCGT123050.042.620596
ATCGGAA235800.040.8352994
CCGCGTT129250.040.648847
AGACCGC131050.040.314514
CGCGTTC140500.037.6312878
GGCGTAC77500.036.5357551
GACCGCG148050.035.6140025
GTACGGA78100.034.9669084
CAGACCG161500.032.8880423
TACGGAA84900.032.1591645
GATCGGA329150.031.96669494
TATACCC992600.030.63144594
GCGTTCT174000.030.3603129
GGAGTCT1157750.027.3679123
GAGTCTT1177000.026.9562474
TGGAGTC1190350.026.611772
AGTCTTG1235050.025.8776365
Produced by FastQC (version 0.11.5)