FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004723397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57801679
Sequences flagged as poor quality0
Sequence length100
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC8835431.5285767044933072No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG5670680.9810580069828075No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC5326850.9215735757433621No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT4331750.749415946896629No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC4240840.733688030065701No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG4182460.723587977435742No Hit
CGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCG2865770.49579355644669076No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC2761390.4777352574827454No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG2070080.35813492545778813No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG1957720.3386960437602513No Hit
CCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGA1909590.3303692960199305No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC1891490.327237899092862No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG1803290.3119788267742188No Hit
CCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGG1708770.29562636061142794No Hit
CCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGG1706260.2951921171701604No Hit
CACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGG1533460.26529679181118593No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG1456410.251966729201759No Hit
CGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTA1450210.2508940960002217No Hit
GGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA1386700.23990652589866812No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG1327630.22968709957369923No Hit
CGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAAC1302140.22527719307254032No Hit
TCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC1283820.22210773496735275No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1261050.21816840303202958No Hit
CCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGG1230800.21293499104065816No Hit
CGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCA1186570.2052829641851753No Hit
GCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCC1176400.203523499723944No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC1149090.19879872347652738No Hit
CCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCC1095960.18960694896077326No Hit
CGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCCCCGCACGCACGCGC1094150.1892938092680664No Hit
CCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTC1081390.18708626093716066No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT1081300.1870706904552029No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1070110.18513476053178315No Hit
GGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCC1047950.18130096186306283No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCC1031670.17848443468225203No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG1022940.17697409793234553No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC1017750.17607620013944578No Hit
CCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGC989020.1711057562878061No Hit
CCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCT975300.16873212281601715No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGA944350.16337760707608512No Hit
CCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGC881340.1524765396520748No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTA874870.15135719500466413No Hit
TTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGA867530.15008733569832805No Hit
GGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCC855200.14795417967010957No Hit
GCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCC840790.14546117250331084No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGG835050.14446812176511342No Hit
GTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCAT831220.14380551125513155No Hit
GGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGAC827300.14312733026319183No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC822930.14237129686146313No Hit
CCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGC822120.14223116252384294No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC792020.13702370133573455No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG784840.13578152288621234No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT783480.1355462356032945No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC778660.13471234979177682No Hit
CGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTG763340.13206190775184923No Hit
GACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGCCCC744870.12886649884339865No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCAC743770.12867619295280333No Hit
CGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCC741980.1283665133671982No Hit
GGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGAC734170.1270153415439714No Hit
CCCGCACGCACGCGCGCGCGCGCGCGCGGACAAACCCTTGTGTCGAGGGC732030.12664511008408597No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC722070.12492197674742285No Hit
CCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAA703030.12162795478657289No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC694180.12009685739405597No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC679470.11755194862073123No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG664100.11489285631304932No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCG660290.11423370591016914No Hit
GCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGG659430.11408492130479463No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGG654600.11324930543972606No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT653610.11307803013819027No Hit
GGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGC653040.11297941708579089No Hit
GGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGG648080.11212131052456106No Hit
GAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACG644450.1114933010855965No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG643150.11126839412398384No Hit
GTGTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGG632650.10945183789557392No Hit
GGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACC623130.10780482691514896No Hit
CCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCAC622970.10777714605833509No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG621180.10746746647272998No Hit
GCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCG604700.10461633822090184No Hit
CCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACG597400.10335339912876924No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC596530.10320288446984385No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA590660.10218734303548517No Hit
CGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCG582170.10071852757079945No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCT582120.10070987730304512No Hit
TGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAG578290.10004726679306324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)