FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20347623
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC78850.031.0539151
GGCGCGT39200.030.2130621
GGCGATC26650.028.7455541
CGACCCG32600.024.6522563
GCGTGCC108350.022.859142
CGTGCGG38550.021.3288258
GTCGACC38800.021.3187271
CCGTGCG37400.021.2308467
GCGCGTG58000.020.7438872
TCGACCC39800.020.428722
CGATCTG43250.018.473153
GGTCGAC24150.017.514955
GCGATCT47950.016.074352
CGTGCCT156050.015.9018753
GTGCGGA54700.015.4610699
ACCCGTG54450.015.1051035
ACGCGCG37600.014.80771632-33
GACCCGT60150.013.4390914
AGGTCGA34950.012.9092554
CGTAGGG38600.012.48034180-81
Produced by FastQC (version 0.11.5)