FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22211921
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG301620.13579194703600828No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT288520.12989421311195912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC50750.041.197251
GGCGATC27150.029.7647931
GGCGCGT19050.028.6093041
GCGTGCC81550.026.0409492
GCGATCT34850.023.727512
ACTCCGC33050.022.4598775
CGTGCCT110750.019.1324773
ACGCCGC14350.018.6623042
GCGCGTG32100.018.0028972
CGCGAGT6050.017.861451
CACGCCG15400.017.695041
CGATCTG52850.016.3572863
ACGCACG14450.015.6051894
GACTCCG50350.015.3026334
TCACCCC223450.015.075693
GCTGCGA76850.014.7942614
GGTGTCG73700.014.7890189
GCGACAT76500.014.7391217
TGCGACA76850.014.5497276
GTCACCC218000.014.5044372
Produced by FastQC (version 0.11.5)