FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14455157
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG253830.1755982311364726No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT174910.12100179887357847No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT156280.10811366490173714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC62100.046.6816021
GGCGCGT23050.038.5250321
GCGTGCC81000.035.963122
GGCGATC19900.033.2903371
GCGCGTG32600.027.6716022
CGTGCCT106750.027.4630893
GCGATCT23850.027.1857552
CGCGGGT5650.020.7894861
CGATCTG35500.018.7928493
GCTGCGA64250.018.3541074
TGGCGCG16900.018.348812
CCTGTAG169200.017.6044127
GCCTGTA174700.017.4535776
GGCTGCG68100.017.3854563
TGTAGTC178000.016.5497789
TCACCCC175650.015.807823
CTGCGAC75150.015.6919665
ACCCCAT182350.015.2270015
GCGACAT77700.015.0560487
ACTCCGC14050.015.0476545
Produced by FastQC (version 0.11.5)