FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20287578
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG753520.3714193976235113No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT512390.252563415899128No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT429830.21186856311778568No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG321670.1585551513344767No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA315880.15570118818520376No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA310640.15311832688948873No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG292120.1439895881115035No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC251200.12381961020679748No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG248730.1226021164280921No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC248050.12226693595460236No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA244350.12044315984884937No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG228830.11279315845390712No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT219930.10840623755087966No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC217150.10703594090925984No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC211110.10405874964473334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC48350.049.182731
CGCGTGC167950.042.644551
GGCGCGT66950.041.4854161
GCGATCT63750.037.375352
GCGTGCC206250.034.6571462
GCGCGTG95000.029.5825162
CGATCTG84050.029.0570263
CGTGCCT256750.027.8779033
ACGCCGC19000.020.5296732
CACGCCG18950.020.3358921
CCTGTAG360900.020.1320487
GCCTGTA369850.020.0131826
CGCGGTG20550.019.4386331
TCACCCC388150.019.2027283
GTGCCTG377200.019.199874
GCTGCGA164600.018.8036064
CGCGAGT7000.018.79831
TGGCGCG45200.018.7150762
GTCACCC398100.018.3095282
GGCTGCG170600.018.2248743
Produced by FastQC (version 0.11.5)