FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14502342
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT789020.5440638484459959No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT420680.2900772854481021No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT357370.24642226752065285No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA264650.18248776645868647No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG234940.16200142018440886No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA182000.12549697145467953No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC150270.1036177467060148No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC147160.10147326549049802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG22850.037.8747561
TGGAGTC253300.032.4396322
TGCGCGG20600.030.771947
CCGCGTT8900.030.6003487
GGAGTCT270200.030.3058973
ACCGCGT8900.030.0727566
GAGTCTT292300.028.1108064
CGCGGAC22050.028.1095379
GTGCGCG23000.027.5609576
GACCGCG10250.026.6143785
AGTCTTG317750.026.4959625
GTCTTGG343150.024.534926
CGCGTTC11600.024.2874368
CTGGAGT387250.023.96371
TCTTGGA382850.022.1133967
GCGTAAC21400.021.88260826-27
TCGCGCG13400.020.3241149
CTTGGAA407400.020.319818
TTGGAAG412700.020.1840139
GCGCGGA31000.020.1455128
Produced by FastQC (version 0.11.5)