FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14502342
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG417440.28784316353868916No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG280840.19365148056775933No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA269770.18601823071059834No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT225960.15580931686757904No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA217120.14971374968263748No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA204380.1409289616808099No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG191840.13228208243882264No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG157660.10871347538211414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC28200.045.83981
GCGATCT37950.033.93882
GGCGCGT15000.033.218021
CGCGTGC34900.031.2479631
CGATCTG54450.024.1722093
GCGCGTG21600.023.0679912
GCGTGCC50650.022.0879362
GCTGCGA105300.018.614974
GGCTGCG110450.017.9173013
CGTAGGG71700.017.52860580-81
TCACCCC256900.017.3094483
TGCGACA111350.017.2655456
GTCACCC249500.017.1446462
GCGACAT115200.016.7701327
CTGCGAC118550.016.613735
CGTGCCT68200.016.4728853
ACCCCAT261800.016.356995
ACGTAGG78600.016.2892780-81
GAACGTA77600.016.28682178-79
AACGTAG77150.016.19878278-79
Produced by FastQC (version 0.11.5)