FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14844417
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA307020.2068252326783868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG22550.032.75191
GTGCGCG17600.022.6799936
TGCGCGG17450.022.6059077
CGCGGAC17000.022.3758039
GGAGTGC194800.018.545795
CGCACTA46850.017.149778-79
GAGTGCA222900.016.4120966
CGATCGG47200.016.3989166-67
TCGCGCG11600.016.1936429
CGCGATC139950.015.90848628-29
GCGTAAC16800.015.82541726-27
GGCGCGA140800.015.65751826-27
GTGCAGT239100.015.4572368
GCGCGGA27450.015.2259478
CACCCGA74100.015.03518694
CGATCCC149300.014.93181130-31
CGGACTT28300.014.78366994
AGTGCAG256950.014.5845297
GCGCGAT137700.014.48931826-27
GGTGCGC27800.014.3829255
Produced by FastQC (version 0.11.5)