FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19380406
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG802730.41419668917152713No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT451120.23277118136740788No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT443050.22860718191352647No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA390360.20141992897362418No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT324730.16755582932576335No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA305520.1576437562763133No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG301500.15556949632530917No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA286920.14804643411495094No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG272680.14069880682582192No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC265640.13706627198625249No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG252390.13022946990893794No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG211890.10933207488016505No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC202510.10449213499448876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC48650.050.990481
CGCGTGC161500.047.099071
GCGTGCC186400.040.883022
GCGATCT65100.038.105792
GGCGCGT59750.037.4288981
CGTGCCT248600.030.6687473
CGATCTG89650.027.9814113
GCGCGTG81850.027.5524712
CGCGGTG19000.023.9859091
GCCTGTA360500.021.6051796
GCTGCGA178700.021.5034124
CCTGTAG359950.021.1814147
GGCTGCG182750.021.1553923
GTGCCTG375850.020.510334
CGCGAGT7600.019.7821921
TGTAGTC393650.019.478269
TGCGACA201700.018.9582026
TGCCTGT411400.018.8635925
GTCACCC420450.018.6612242
TCACCCC426750.018.6253933
Produced by FastQC (version 0.11.5)