FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15565943
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA590930.3796300680273595No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT415450.266896775865105No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT286860.1842869397633025No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA263710.16941472803799937No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG256800.16497554950573826No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC248430.15959842587114703No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG223580.14363408628696636No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC192750.12382802635214583No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA168890.10849969063872326No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC159870.10270498870514945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG34750.041.3718871
TGGAGTC167450.024.8032512
GGAGTCT183150.022.8773653
TGCGCGG12350.022.820517
CGCGGAC12150.022.8094089
ACCGCGT10900.022.4089096
GGAGTGC275550.022.280345
CGCGTTC10800.021.3114688
GTGCGCG13700.021.2576376
TCGCGCG7550.021.1529169
CCGCGTT11550.020.3342917
ACCGAAG124200.020.32048294
GAGTCTT208800.020.0669334
GTGCAGT311950.019.9061628
GAGTGCA324700.019.3126956
TGGAGTG319850.019.2385164
CTCGCTA18200.018.8447421
AGTCTTG229600.018.6377355
GTCTTGG234550.018.023996
AGTGCAG356900.017.6622927
Produced by FastQC (version 0.11.5)