FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15226640
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG593520.3897905250271892No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT384300.2523866066315353No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT360200.23655908329086392No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC312620.20531121770791194No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA293300.19262292928709157No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA278860.1831395501568304No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG273940.17990837111798794No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA248550.16323364839518106No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG221540.1454950008669017No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG210760.1384153037045599No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC210730.13839560139334744No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG207270.13612326816684445No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC172560.1133276940940352No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC170960.11227690416270432No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT158000.1037655057189242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC40350.053.704641
GGCGCGT56100.043.5707361
CGCGTGC131750.041.3511121
GCGATCT55100.039.242732
GCGTGCC159850.034.11132
GCGCGTG76300.032.343552
CGATCTG70150.030.9572943
CGTGCCT197700.027.5803643
ACTCCGC21150.022.2247525
GCCTGTA281650.020.0599176
CCTGTAG280100.019.6171467
GCTGCGA135400.019.5797754
GTGCCTG285050.019.2605674
GGCTGCG139800.019.1653333
TGGCGCG37500.018.8023852
TCACCCC318650.018.5130713
GGGCGAT38100.018.5063461
GTCACCC319050.017.9596522
TGCCTGT312000.017.8831235
TGTCACC324500.017.8319051
Produced by FastQC (version 0.11.5)