FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10643444
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA252340.2370849134922869No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT169630.15937510452443776No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG124990.11743379304668677No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC120560.11327160644618414No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA118520.11135493360983531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG18850.035.6417771
TCGCGCG6250.024.8031839
CGCGGAC7350.020.4520179
GGAGTGC140650.019.4716325
CTCGCTA11500.018.7929381
ACCGAAG44850.018.40066394
TGCGCGG8200.018.3319917
TGGAGTC104600.018.325812
GGAGTCT100950.017.9154573
CGCACTA44700.017.7709678-79
GTGCAGT165850.017.1361568
TATACCC98500.016.94825494
GAGTGCA168800.016.5862166
GTGCGCG9350.016.5796686
TCGAGTC15550.016.3131143
TCGATCC14300.016.0988462
TTGCGTC20200.016.0461648
GAGTCTT112450.015.8744124
CGATCGG48950.015.75868466-67
TTTGCGT25550.015.6279337
Produced by FastQC (version 0.11.5)