FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17573449
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1375120.7824986432657585No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA753310.42866371877256426No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT692820.394242473404054No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT508200.28918626047738266No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG458180.2607228666381881No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA441970.25149872401257145No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG425940.24237700863387718No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA381290.21696936099453215No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC270810.15410179299464777No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG267900.15244588583607008No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC258110.1468749816840166No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC240120.13663794739439025No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC187760.10684299934520537No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC187270.10656416961747235No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT183880.10463512313376845No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC183090.10418558132783155No Hit
CGAAGACCGGTCCTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCG181770.10343444818373446No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG177510.10101033667323928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG65650.042.4155771
TGGAGTC368650.039.707152
GGAGTCT404250.036.3382533
ACCGCGT11700.034.9152266
GAGTCTT444050.033.19774
AGTCTTG465500.032.2237245
GACCGCG12300.032.1229255
CCGCGTT12500.031.9295567
GTCTTGG489550.030.6063296
TCTTGGA539300.027.9485057
CTCGCTA36100.026.7106881
CTGGAGT771750.025.970081
TGCGCGG40400.025.9183567
CTTGGAA578150.025.7131678
TCGCTAT38550.025.5014722
AGACCGC16300.025.1057114
GTGCGCG42800.025.013396
CGCGGAC41750.024.9678829
TTGGAAG612850.024.2802549
GCTATGT127550.022.2740822
Produced by FastQC (version 0.11.5)