FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16129907
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT372760.23109866659491587No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT240780.14927550419230562No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA213980.1326604052955792No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT169070.10481771531602754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG25150.035.5351451
TGCGCGG33700.028.985047
GTGCGCG35550.027.4763376
CGCGGAC35100.027.2936869
TGGAGTC187650.023.0361252
GGAGTCT186350.022.9697973
GCGTAAC32100.022.57691226-27
GAGTCTT192650.022.365074
CGGACTT46550.021.10791294
GCGCGGA47800.020.6313578
AGTCTTG223850.020.5928065
GGTGCGC50600.019.5215935
GTCTTGG228850.019.4122266
GCGGACT38450.019.06490792-93
CGGGATA38250.018.80383176-77
ACCGCGT11500.017.9675946
GGATACG40600.017.59172878-79
CTGCGTA43150.017.22470724-25
ATACGCT41850.017.17220380-81
CCGCGTT12100.016.6887427
Produced by FastQC (version 0.11.5)