FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18278495
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA316020.17289169595199166No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT272300.14897287769042256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG29100.037.9519271
ACCGAAG97700.019.09691694
TGCGCGG16450.018.854637
TCGCGCG13850.017.9829679
TGGAGTC178250.016.7149722
GGAGTCT179400.016.6862263
CGCGGAC18900.016.410339
GGAGTGC226800.016.0167795
GAGTCTT187000.015.9326784
GTCTTGG194750.015.85366
CGCACTA65200.015.82214978-79
GTGCGCG19250.015.6238926
AGTCTTG209550.015.4066375
CGATCGG66400.015.21836466-67
AGCGCAC74600.014.30030976-77
GGCGCGA148550.014.235908526-27
CTCGCTA17500.014.2330111
TATACCC197500.014.15849794
CGCGATC151000.014.08294628-29
GAGTGCA259950.014.0465986
Produced by FastQC (version 0.11.5)