FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18278495
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC47950.036.830741
GGCGCGT18700.029.6381861
GCGTGCC66400.026.8090342
GGCGATC21600.025.8764341
GCGATCT29150.019.496512
GCGCGTG30400.019.4673562
CGTGCCT97900.018.663523
CGATCTG32100.018.144433
ACTCCGC19350.017.059175
CGTAGGG28850.015.06457580-81
ACGTAGG35850.012.45074780-81
CGTAGCC83950.012.03787794
TCACCCC149800.011.915123
GAACGTA38000.011.87738578-79
GCGACAT47650.011.8757317
TCCGCTG35300.011.7557417
AACGTAG38300.011.6002278-79
TGCGACA48550.011.4612936
ATCGTAG107700.011.30358394
CGGATCG138100.011.2999594
Produced by FastQC (version 0.11.5)