FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004723512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51550390
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2779430.5391675989260217No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1391840.26999601748890745No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1235530.23967422942872013No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT1081920.20987620074261318No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1073080.2081613737548833No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT1054000.20446014084471525No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC735130.14260415876582117TruSeq Adapter, Index 8 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA665960.12918621954169504No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA532650.10332608540885918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)