FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004723513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51550390
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1194960.23180425987077888No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT971740.188502938581066No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG896830.17397152572463565No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG777290.15078256439960977No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT723330.14031513631613649Illumina Single End PCR Primer 1 (100% over 50bp)
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT584990.11347925786788422No Hit
GCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCG582770.11304861127141813No Hit
GCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGC529720.10275770949550528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)