FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17894896
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT23000.035.9390831
CGCGTGC47200.028.2591081
CGCGGGT10550.027.600781
GCGCGTG32500.025.8673422
GCGTGCC66150.020.3767152
GGCGATC21750.017.0588571
GCGATCT26050.014.0627092
TCGTCGG16550.014.04753814-15
TGGCGCG19950.013.8901123
CGTGCCT101950.013.5443073
GCGGGTC23150.012.98407652
ATCGTCG17650.012.77290214-15
CGATCTG30700.012.2390663
CGTCGGG19150.011.527266516-17
GATCGTA95950.011.31444394
CGGATCG114200.011.27588694
CGGGTCT27900.011.1105723
AGCGCGA16950.010.53610494
CGAAGCT92200.010.29642894
CGTAGCC69400.010.29319794
Produced by FastQC (version 0.11.5)