FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21480927
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1896930.8830764147189738No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1781480.8293310619229795No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT1285240.5983168231054461No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG934400.43499053835060286No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG879120.40925608098756633No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA818360.38097052329259345No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT733750.34158209280260576No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA677340.3153215873784218No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC659700.3071096512734297No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC575150.26774915253890114No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG472830.22011619889588563No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC431830.20102949933212844No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC390240.18166813750635621No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC354640.16509529593392316No Hit
AGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTG320720.14930454351434647No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC309740.14419303226532076No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC285700.13300170891135193No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA271030.12617239470158806No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC262540.12222005130411737No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC256000.11917548995906926No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC242810.11303515905063129No Hit
CGAAGACCGGTCCTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCG240620.11201564997637206No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA229690.10692741519022898No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG227940.10611273898933692No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC224970.10473011709410865No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA221750.10323111288446724No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA221700.1032078364215846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG122850.050.9909441
ACCGCGT14600.046.9933136
GACCGCG15050.046.212585
CCGCGTT15450.044.1036537
TGGAGTC478050.039.4976842
GGAGTCT525250.035.930453
GAGTCTT579550.032.5396044
AGACCGC21900.032.401634
CGCGTTC22200.031.9633248
GCTATGT199050.031.730152
AGTCTTG629400.030.350715
CTCGCTA52600.029.1252671
GTCTTGG655900.029.0385556
TATGCGA27350.029.0381169
TTATGCG27050.028.4907938
TGCGCGG51150.028.2969827
CGCGGAC53100.027.1695799
GTGCGCG54200.026.791396
TCGCTAT59350.025.970972
TCTTGGA740550.025.953987
Produced by FastQC (version 0.11.5)