FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24109801
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG283670.1176575451618203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC46500.034.6677551
GGCGATC36800.034.610351
GGCGCGT22400.026.4366911
GCGATCT53200.024.1176832
CGATCTG58250.022.0266573
GCGTGCC78300.021.1283442
CGCGAGT8100.019.727791
GCGCGTG31500.019.396232
TGCGACA93600.016.2668446
GCTGCGA95500.015.8940034
CGTGCCT104750.015.7932133
GCGACAT96200.015.7294997
GGGCGAT30150.014.9647161
CTGCGAC105450.014.6616695
GGCTGCG108900.014.069273
CGACATC114600.013.6140298
ACTCCGC27200.013.4759635
TCACCCC249450.012.4914223
ACGCACG20900.012.14125394
GTCACCC252500.011.8566672
Produced by FastQC (version 0.11.5)