FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23753267
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG281350.11844686459340519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC36150.037.8220861
CGCGTGC48800.034.0835041
GCGATCT52700.025.766062
GGCGCGT22200.024.1275181
CGATCTG59750.023.3547733
GCGTGCC77850.022.0893652
CGCGAGT8150.017.8716581
TGCGACA93550.016.9246
GGGCGAT28450.016.8452971
GCGACAT95250.016.6712327
GCTGCGA96700.016.3727024
CGTGCCT104800.016.3639723
GCGCGTG34000.016.1684252
ACTCCGC26850.015.7476265
CTGCGAC102950.015.4699985
CGACATC110450.014.717218
ACGCACG20050.014.526937594
GGCTGCG109700.014.348133
CGACCTA14700.014.0621674
CTACGCA22650.013.6896028
Produced by FastQC (version 0.11.5)