FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15056395
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT437010.290248761406698No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA307710.20437163079209866No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT236060.1567838782125469No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG177390.11781704717497117No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT169300.11244391502746841No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG160650.10669884789818544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG27500.038.1431
TGCGCGG21200.024.8022927
TGGAGTC196000.024.7188452
GGAGTCT196900.024.534113
CGCGGAC22500.023.9949159
GAGTCTT207700.023.3717734
GTGCGCG23200.023.0684976
AGTCTTG227950.021.9766435
GTCTTGG242850.020.317356
CTGGAGT379800.018.1189081
TATACCC255950.017.82586594
TCTTGGA285550.017.7891317
GCGTAAC22450.017.51083226-27
GGAGTGC210450.017.1434335
ACCGAAG135650.016.9213194
CTTGGAA286600.016.8229068
CTCGCTA19050.016.5433141
TTGGAAG293750.016.317439
GCGCGGA33350.016.047818
GAGTGCA233550.015.6990576
Produced by FastQC (version 0.11.5)