FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21470951
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT436640.2033631393411498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG5500.035.0966769
CGCTATG24050.028.326811
CCGCGTT10250.021.1287947
ACCGCGT10300.020.9825026
TGGAGTC221550.020.549242
GGAGTCT221200.020.3480643
GACCGCG10950.019.7369655
AGTCTTG253100.019.0826035
GAGTCTT244300.018.9432954
GTCTTGG253950.018.8152266
TATACCC231800.018.6690194
CGCGTTC12050.017.582088
CGCGCTA1350.00989794817.4373729
CCTCGCG11550.016.7125457
ACCGAAG98950.016.05003594
CTCGCGC12200.015.8222718
TCTTGGA305100.015.7860767
CACCCGA88350.014.99745894
CTGGAGT390600.014.3981491
CCCTACG154750.014.11944424-25
Produced by FastQC (version 0.11.5)