FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20124069
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG317260.15765201361613299No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA223640.11113060683701692No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT208360.10353770899910947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC86400.037.203361
GGCGCGT38450.029.9439851
GCGTGCC115900.028.098712
GGCGATC25300.027.1189061
CGTGCCT152250.021.5444163
GCGCGTG56400.020.9137172
CGACCCG19300.018.7486523
GGTCGAC15450.018.2502615
GCGATCT40000.018.0924472
GCGACAT80950.017.7032387
GCTGCGA82000.017.3646664
TGCGACA82450.017.2702356
CCGTGCG25000.016.7270627
CGTAGGG67500.016.70975180-81
TCACCCC219150.016.2755973
ACGTAGG72350.015.84943680-81
CTGCGAC91950.015.8436875
GAACGTA72750.015.63456778-79
AACGTAG72650.015.59139478-79
GTCGACC26050.015.5142411
Produced by FastQC (version 0.11.5)