FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19324813
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG235740.12198824381896994No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT224030.1159286767742591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC74250.045.924221
GCGTGCC98550.034.8863072
GGCGCGT28600.033.33741
GGCGATC24450.027.2779141
GCGCGTG38550.024.8545742
CGTGCCT141300.024.2663083
GCGATCT32950.019.6708982
GCGACAT75250.016.5436297
CCTGTAG214700.016.2749447
GCTGCGA80100.015.7773264
CGTAGGG51000.015.70751980-81
GCCTGTA227700.015.6977046
CGATCTG43000.015.6203443
TGCGACA80950.015.3786076
TGTAGTC230450.015.06032859
GGCTGCG84250.014.9970343
GAACGTA55600.014.45924778-79
AACGTAG56650.014.35722778-79
TCACCCC200900.014.1214723
ACGTAGG58100.013.78801280-81
Produced by FastQC (version 0.11.5)