FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10475874
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT749540.7154916143512227No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT252380.24091545965520392No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA231190.2206880304211372No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT219950.20995861538617208No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA207350.19793097931494785No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG152600.1456680368626045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTC193450.039.367662
TCGCGCG4350.036.694229
GGAGTCT214800.035.297353
CCGCGTT3200.033.743177
GACCGCG3500.032.192245
GAGTCTT240350.031.7407574
AGTCTTG253500.030.3905565
GTCTTGG255150.030.0652286
CGCGTTC4150.029.412598
CTGGAGT299550.028.3566151
CTCGCGC5700.028.0034858
CCTCGCG5900.027.0542147
ACCGCGT4050.026.6612726
TCTTGGA294100.026.2590437
CGCTATG14300.025.6261791
TTGGAAG306650.024.847559
TATACCC299150.024.51895994
CTTGGAA310250.024.4381738
TGCGCGG7900.022.582119
ACCGAAG121200.020.91277794
Produced by FastQC (version 0.11.5)