FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7671947
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT338830.4416479936579332No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA272420.3550858732470389No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG170230.22188630865150658No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG149450.19480061580196004No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA136340.17771238513509022No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC126510.16489947076016037No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT124140.16181029404921593No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA106660.1390259864933895No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT104070.1356500507628637No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC90910.11849664759154357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG20300.039.80831
TGGAGTC116700.029.1882742
GGAGTCT121200.028.5272453
GAGTCTT132750.025.832894
AGTCTTG144350.024.5705475
GTCTTGG146900.023.536286
CTCGCTA10800.023.05651
TATACCC152100.021.33463994
CTGGAGT260100.019.3640291
CTTGGAA178550.019.1800388
TTGGAAG178300.019.1278939
TCTTGGA185300.019.0644477
TCGCTAT12700.018.8672122
GCTATGT44950.018.2915122
GGAGTGC152850.018.1945325
CGCGGAC9250.017.2670848
GAGTGCA162150.017.23786
TGCGCGG9400.016.9915456
ACCGAAG69250.016.891194
CGCACTA57000.016.4919578-79
Produced by FastQC (version 0.11.5)