FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9816204
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT707500.7207470423393809No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT249800.2544771889418761No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA224360.22856085712970106No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT211570.21553138056218066No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA203200.20700466290227873No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG142840.1455144982724483No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG117930.12013809003969356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTC190450.037.0618172
GGAGTCT207400.034.123533
GAGTCTT228300.031.184754
AGTCTTG241350.030.0632255
GTCTTGG241950.029.6779066
CGCGGAC10200.029.0252489
CGCTATG17300.027.707291
CTGGAGT304850.026.7764841
TCGCGCG5400.026.1073639
TCTTGGA287300.025.51677
TGCGCGG11400.024.7332947
TTGGAAG297050.024.1254739
CTTGGAA297050.024.014618
GTGCGCG11950.023.5949426
TATACCC295000.022.83059594
GACCGCG4250.018.7973045
CCTCGCG7250.018.79737
CTCGCGC7550.018.6727228
ACCGAAG124150.018.5120894
CACCCGA53550.018.4311794
Produced by FastQC (version 0.11.5)