FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22549055
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG302940.1343470934813011No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT295730.1311496202390743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC48450.040.354311
GGCGATC25400.029.050681
GCGTGCC79500.024.9479432
GGCGCGT18100.024.9277481
GCGATCT35700.021.1956672
ACTCCGC32600.020.3253945
CGTGCCT107400.018.5545753
CGCGAGT5450.018.109781
ACGCCGC16750.017.957862
CACGCCG18500.016.005131
GGTGTCG75700.015.5815439
GCGCGTG31350.015.4414862
CGATCTG53350.015.0643753
TCACCCC234400.014.5167843
GACTCCG48250.014.4145764
CGGACAT30600.014.2840691
GCTGCGA78000.014.2787824
GTCACCC222050.013.8637262
TGCGACA79750.013.7886156
ATCCCCG217150.013.6554186
Produced by FastQC (version 0.11.5)