FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18806185
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG757520.4028036520963714No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT458310.24370173961385577No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA443170.23565119666747936No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG379390.2017368222209874No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA354810.18866665408215436No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA307670.16360043251728087No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG262480.13957110386822208No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG248730.13225967946183662No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC234600.12474619387185652No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC219130.11652017673972687No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCA212770.11313831061430055No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT203390.10815058981925361No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC198910.10576839481266402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC38750.051.3869061
CGCGTGC68550.041.448251
GGCGCGT25300.039.352621
GCGATCT55600.035.4757542
GCGTGCC96150.029.7946072
CGATCTG75300.026.6950863
GCGCGTG39600.025.9720422
CGTGCCT126450.022.7308243
ACTCCGC22850.020.8453375
TCACCCC387850.020.5857963
GTCACCC377200.020.493462
AGCTTCC211750.020.39767894
GGCTGCG149800.020.3477063
TGTCACC386750.020.1696151
GCTGCGA150950.019.901294
ACCCCAT401600.019.8457165
CCCATTG398450.019.6949837
CCCCATT405650.019.3801846
CACCCCA433400.018.6072484
CGTAGGG119800.018.49011880-81
Produced by FastQC (version 0.11.5)